Tsunekiyo Fujisawa

There's a war going on in ancient Kyoto. A war fought in shadows, between the forces of the Shogunate's armed enforcers, the Shinsengumi, and the vampire-like Ronin warriors known as the Rasetsu or Furies. And trapped in the middle is a young woman disguised as a man. Seeking her missing father, Chizuru Yukimura comes to Kyoto only to find her fate forever intertwined with the destinies of Shinsengumi Vice commander Toshizo Hijikata and his elite force of swordsmen. Because Chizuru's father has created a secret elixir that enhances the user's strength, speed and healing, and the furies will do anything and kill anyone in order to control that power!

The second part of the retelling of the first and second seasons of the anime.

Yukimura Chizuru is the daughter of a doctor who works in Edo; her father leaves Edo to work as a volunteer doctor and moves to Kyoto without his daughter. As time passes by, Chizuru starts worrying about losing contact with her father, so she decides to go to Kyoto in search of him. On the way, Chizuru is attacked by few criminals and witnesses a fight between an oni and the Shinsengumi. Taking her into custody and saving her, the Shinsengumi debate on what to do with Chizuru when they discover that she is the daughter of the doctor they are also looking for. So they decide to become Chizuru's protectors and help her look for her father (the doctor). Lots of events happen while she stays with the Shinsengumi, as they discover mysterious secrets and also fight against the Bakumatsu group.